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Western blot All lanes: CHIA antibody at 0.74ug/ml + Mouse stomach tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 41, 34 kDa Observed band size: 53 kDa
Western blot All lanes: CHIA antibody at 0.74ug/ml + Mouse stomach tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 41, 34 kDa Observed band size: 53 kDa
Western blot All lanes: CHIA antibody at 0.74ug/ml + Mouse stomach tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 41, 34 kDa Observed band size: 53 kDa

CHIA Antibody

CSB-PA874858ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCHIA
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Overview

  • Supplier
    Cusabio
  • Product Name
    CHIA Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27159
  • Target name
    CHIA
  • Target description
    chitinase acidic
  • Target synonyms
    acidic mammalian chitinase; AMCASE; CHIT2; lung-specific protein TSA1902; TSA1902
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BZP6
  • Protein Name
    Acidic mammalian chitinase
  • Scientific Description
    Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMCase/CHIA using anti-AMCase/CHIA antibody (A00437-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMCase/CHIA antigen affinity purified polyclonal antibody (Catalog # A00437-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMCase/CHIA at approximately 55 kDa. The expected band size for AMCase/CHIA is at 55 kDa.
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