ICC/IF analysis of COS7 cells transiently transfected with Chk2 plasmid using GTX84685 Chk2 antibody [2F4].
![ICC/IF analysis of HT29 cells using GTX84685 Chk2 antibody [2F4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84685/GTX84685_1189_ICCIF_w_23061420_600.webp "ICC/IF analysis of HT29 cells using GTX84685 Chk2 antibody [2F4].")
![WB analysis of various samples using GTX84685 Chk2 antibody [2F4]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84685/GTX84685_3559_WB_w_23061420_843.webp "WB analysis of various samples using GTX84685 Chk2 antibody [2F4]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon) using GTX84685 Chk2 antibody [2F4]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84685/GTX84685_4165_WB_w_23061420_165.webp "WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon) using GTX84685 Chk2 antibody [2F4]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of wild type and Chk2 knock out HeLa cell lysate(10microg per lane) using GTX84685 Chk2 antibody [2F4]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84685/GTX84685_4554_20181228_WB_w_23061420_619.webp "WB analysis of wild type and Chk2 knock out HeLa cell lysate(10microg per lane) using GTX84685 Chk2 antibody [2F4]. Dilution : 1:500")
![WB analysis of HEK293T cells transfected with Chk2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84685 Chk2 antibody [2F4]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84685/GTX84685_4555_WB_w_23061420_920.webp "WB analysis of HEK293T cells transfected with Chk2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84685 Chk2 antibody [2F4]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX84685 Chk2 antibody [2F4]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84685/GTX84685_4557_WB_w_23061420_881.webp "WB analysis of various cell lines using GTX84685 Chk2 antibody [2F4]. Loading : 35 ug per lane")
![FACS analysis of HEK293T cells transfected with either Chk2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84685 Chk2 antibody [2F4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84685/GTX84685_504_FACS_w_23061420_758.webp "FACS analysis of HEK293T cells transfected with either Chk2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84685 Chk2 antibody [2F4].")
ICC/IF analysis of COS7 cells transiently transfected with Chk2 plasmid using GTX84685 Chk2 antibody [2F4].
Chk2 antibody [2F4]
GTX84685
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetCHEK2
Overview
- SupplierGeneTex
- Product NameChk2 antibody [2F4] - KO/KD, Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:2000. ICC/IF: 1:50-1:100. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2F4
- Concentration1.2 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID11200
- Target nameCHEK2
- Target descriptioncheckpoint kinase 2
- Target synonymsCDS1; cds1 homolog; checkpoint-like protein CHK2; CHK2; CHK2 checkpoint homolog; hCds1; HuCds1; LFS2; PP1425; RAD53; serine/threonine-protein kinase Chk2
- HostMouse
- IsotypeIgG2b
- Protein IDO96017
- Protein NameSerine/threonine-protein kinase Chk2
- Scientific DescriptionRegulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on Ser-216, preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at Thr-18 and Ser-20.
- ReactivityCanine, Human, Monkey, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203