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ProductsBack/Chk2 (CHEK2) Mouse Monoclonal Antibody [Clone ID: OTI5C4]
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and CHEK2-Knockout hela cells (KO, Cat# LC810324) were separated by SDS-PAGE and immunoblotted with anti-CHEK2 monoclonal antibody TA500398. Then the blotted membrane was stripped and reprobed with anti-β-actin (TA811000) as a loading control (1:500).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and CHEK2-Knockout hela cells (KO, Cat# LC810324) were separated by SDS-PAGE and immunoblotted with anti-CHEK2 monoclonal antibody TA500398. Then the blotted membrane was stripped and reprobed with anti-β-actin (TA811000) as a loading control (1:500).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and CHEK2-Knockout hela cells (KO, Cat# LC810324) were separated by SDS-PAGE and immunoblotted with anti-CHEK2 monoclonal antibody TA500398. Then the blotted membrane was stripped and reprobed with anti-β-actin (TA811000) as a loading control (1:500).

Chk2 (CHEK2) Mouse Monoclonal Antibody [Clone ID: OTI5C4]

CF500398
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetCHEK2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Chk2 (CHEK2) Mouse Monoclonal Antibody [Clone ID: OTI5C4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5C4
  • Gene ID11200
  • Target name
    CHEK2
  • Target description
    checkpoint kinase 2
  • Target synonyms
    CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, TPDS4, hCds1, serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, cds1 homolog, checkpoint-like protein CHK2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO96017
  • Protein Name
    Serine/threonine-protein kinase Chk2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) CHEK2 mouse monoclonal antibody, clone OTI5C4 (formerly 5C4)
  • Reactivity
    Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of CHEK2 using anti-CHEK2 antibody (A00277-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHEK2 antigen affinity purified polyclonal antibody (Catalog # A00277-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHEK2 at approximately 62 kDa. The expected band size for CHEK2 is at 61 kDa.
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