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Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody

CAU23543
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetCHRNA3
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Overview

  • Supplier
    Biomatik
  • Product Name
    Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1136
  • Target name
    CHRNA3
  • Target description
    cholinergic receptor nicotinic alpha 3 subunit
  • Target synonyms
    BAIPRCK, LNCR2, NACHRA3, PAOD2, neuronal acetylcholine receptor subunit alpha-3, cholinergic receptor, nicotinic alpha 3, cholinergic receptor, nicotinic, alpha 3 (neuronal), cholinergic receptor, nicotinic, alpha polypeptide 3, neuronal nicotinic acetylcholine receptor, alpha3 subunit
  • Host
    Rabbit
  • Protein IDP32297
  • Protein Name
    Neuronal acetylcholine receptor subunit alpha-3
  • Scientific Description
    The Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CHRNA3 using anti-CHRNA3 antibody (A01981-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SK-OV-3 whole cell lysates, Lane 6: human PANC-1 whole cell lysates, Lane 7: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRNA3 antigen affinity purified polyclonal antibody (Catalog # A01981-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHRNA3 at approximately 60KD. The expected band size for CHRNA3 is at 57KD.
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