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Western blot analysis of extracts from Jurkat cells, K562 cells and HT-29 cells, using CHP2 antibody.
Western blot analysis of extracts from Jurkat cells, K562 cells and HT-29 cells, using CHP2 antibody.
Western blot analysis of extracts from Jurkat cells, K562 cells and HT-29 cells, using CHP2 antibody.

CHP2 Antibody

CSB-PA869859
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCHP2
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Overview

  • Supplier
    Cusabio
  • Product Name
    CHP2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID63928
  • Target name
    CHP2
  • Target description
    calcineurin like EF-hand protein 2
  • Target synonyms
    calcineurin B homologous protein 2, hepatocellular carcinoma antigen gene 520, hepatocellular carcinoma-associated antigen 520
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43745
  • Protein Name
    Calcineurin B homologous protein 2
  • Scientific Description
    Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na+/H+ exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus. Loftus B.J., Genomics 60:295-308(1999). Wang Y., J. Immunol. 169:1102-1109(2002). Pang T., J. Biol. Chem. 277:43771-43777(2002).
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CHP2 using anti-CHP2 antibody (A08478-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHP2 antigen affinity purified polyclonal antibody (A08478-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHP2 at approximately 22 kDa. The expected band size for CHP2 is at 22 kDa.
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