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ProductsBack/Chromogranin A antibody [LK2H10] (ready-to-use)
IHC-P analysis of human pancreas tissue using GTX73638 Chromogranin A antibody [LK2H10] (ready-to-use).
IHC-P analysis of human pancreas tissue using GTX73638 Chromogranin A antibody [LK2H10] (ready-to-use).
IHC-P analysis of human pancreas tissue using GTX73638 Chromogranin A antibody [LK2H10] (ready-to-use).

Chromogranin A antibody [LK2H10] (ready-to-use)

GTX73638
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCHGA
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Overview

  • Supplier
    GeneTex
  • Product Name
    Chromogranin A antibody [LK2H10] (ready-to-use)
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    LK2H10
  • Conjugate
    Unconjugated
  • Gene ID1113
  • Target name
    CHGA
  • Target description
    chromogranin A
  • Target synonyms
    CGA, PHE5, PHES, chromogranin-A, SP-I, betagranin (N-terminal fragment of chromogranin A), catestatin, chromofungin, parathyroid secretory protein 1, pituitary secretory protein I
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP10645
  • Protein Name
    Chromogranin-A
  • Scientific Description
    The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Sei Y, Feng J, Zhao X, et al. Polyclonal Crypt Genesis and Development of Familial Small Intestinal Neuroendocrine Tumors. Gastroenterology. 2016,151(1):140-51. doi: 10.1053/j.gastro.2016.03.007
    Read this paper
  • Adam RM, Kim J, Lin J, et al. Heparin-binding epidermal growth factor-like growth factor stimulates androgen-independent prostate tumor growth and antagonizes androgen receptor function. Endocrinology. 2002,143(12):4599-608.
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chromogranin A/CHGA antigen affinity purified polyclonal antibody (Catalog # A01256-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chromogranin A/CHGA at approximately 70 kDa. The expected band size for Chromogranin A/CHGA is at 51 kDa.
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