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Western Blot analysis of A549 cells using Chr-A Polyclonal Antibody
Western Blot analysis of A549 cells using Chr-A Polyclonal Antibody
Western Blot analysis of A549 cells using Chr-A Polyclonal Antibody

CHGA Antibody

CSB-PA001643
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCHGA
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Overview

  • Supplier
    Cusabio
  • Product Name
    CHGA Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1113
  • Target name
    CHGA
  • Target description
    chromogranin A
  • Target synonyms
    CGA, PHE5, PHES, chromogranin-A, SP-I, betagranin (N-terminal fragment of chromogranin A), catestatin, chromofungin, parathyroid secretory protein 1, pituitary secretory protein I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10645
  • Protein Name
    Chromogranin-A
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Chromogranin A/CHGA using anti-Chromogranin A/CHGA antibody (A01256-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chromogranin A/CHGA antigen affinity purified polyclonal antibody (Catalog # A01256-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chromogranin A/CHGA at approximately 70 kDa. The expected band size for Chromogranin A/CHGA is at 51 kDa.
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