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ProductsBack/CIB1 (1-191) Mouse Monoclonal Antibody [Clone ID: 1D1]
Western blot analysis Cell lysates of HT29 (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CIB1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis Cell lysates of HT29 (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CIB1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Western blot analysis Cell lysates of HT29 (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CIB1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

CIB1 (1-191) Mouse Monoclonal Antibody [Clone ID: 1D1]

AM03117PU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetCIB1
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    CIB1 (1-191) Mouse Monoclonal Antibody [Clone ID: 1D1]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1D1
  • Gene ID10519
  • Target name
    CIB1
  • Target description
    calcium and integrin binding 1
  • Target synonyms
    CIB, CIBP, EV3, KIP1, PRKDCIP, SIP2-28, calcium and integrin-binding protein 1, DNA-PK interaction protein, DNA-PKcs-interacting protein, DNA-dependent protein kinase interacting protein, SNK-interacting protein 2-28, calcium and integrin binding 1 (calmyrin), testicular secretory protein Li 9
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ99828
  • Protein Name
    Calcium and integrin-binding protein 1
  • Scientific Description
    CIB1 (1-191) mouse monoclonal antibody, clone 1D1, Aff - Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of CIB1 using anti-CIB1 antibody (PB9489). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse brian tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIB1 antigen affinity purified polyclonal antibody (Catalog # PB9489) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIB1 at approximately 22 kDa. The expected band size for CIB1 is at 27 kDa.
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