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CIT / CRIK / Citron Antibody (aa1925-1975)

LS-C289055
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetCIT
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CIT / CRIK / Citron Antibody (aa1925-1975)
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP (10 µg/mg lysate), WB (1:2000 - 1:10000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11113
  • Target name
    CIT
  • Target description
    citron rho-interacting serine/threonine kinase
  • Target synonyms
    CITK, CRIK, MCPH17, STK21, citron Rho-interacting kinase, citron (rho-interacting, serine/threonine kinase 21), serine/threonine kinase 21, serine/threonine-protein kinase 21
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CIT using anti-CIT antibody (A01331-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIT antigen affinity purified polyclonal antibody (A01331-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIT at approximately 231 kDa. The expected band size for CIT is at 231 kDa.
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