Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4 antibody [N3C3] (GTX120087) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![CKAP4 antibody [N3C3] detects CKAP4 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CKAP4 stained by CKAP4 antibody [N3C3] (GTX120087) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX120087/GTX120087_44482_20220311_ICC_IF_w_23060519_385.webp "CKAP4 antibody [N3C3] detects CKAP4 protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CKAP4 stained by CKAP4 antibody [N3C3] (GTX120087) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![CKAP4 antibody [N3C3] detects CKAP4 protein at cytoplasm and membrane in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CKAP4 antibody [N3C3] (GTX120087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX120087/GTX120087_40373_20150724_IHC-P_w_23060519_995.webp "CKAP4 antibody [N3C3] detects CKAP4 protein at cytoplasm and membrane in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CKAP4 antibody [N3C3] (GTX120087) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![CKAP4 antibody [N3C3] detects CKAP4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CKAP4 stained by CKAP4 antibody [N3C3] (GTX120087) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX120087/GTX120087_44482_20230609_IHC-P_M_23062718_894.webp "CKAP4 antibody [N3C3] detects CKAP4 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CKAP4 stained by CKAP4 antibody [N3C3] (GTX120087) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4 antibody [N3C3] (GTX120087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX120087/GTX120087_44482_20230908_WB_M_R_23091319_414.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4 antibody [N3C3] (GTX120087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4 antibody [N3C3] (GTX120087) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CKAP4 antibody [N3C3]
GTX120087
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCKAP4
Overview
- SupplierGeneTex
- Product NameCKAP4 antibody [N3C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.19 mg/ml
- ConjugateUnconjugated
- Gene ID10970
- Target nameCKAP4
- Target descriptioncytoskeleton associated protein 4
- Target synonymsCLIMP-63, CLIMP63, ERGIC-63, p63, cytoskeleton-associated protein 4, 63 kDa membrane protein, 63-kDa cytoskeleton-linking membrane protein, transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediate compartment, type-II transmembrane protein p63
- HostRabbit
- IsotypeIgG
- Protein IDQ07065
- Protein NameCytoskeleton-associated protein 4
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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