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Western Blot analysis of Jurkat cells using Creatine Kinase M Polyclonal Antibody
Western Blot analysis of Jurkat cells using Creatine Kinase M Polyclonal Antibody
Western Blot analysis of Jurkat cells using Creatine Kinase M Polyclonal Antibody

CKM Antibody

CSB-PA001779
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCKM
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Overview

  • Supplier
    Cusabio
  • Product Name
    CKM Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1158
  • Target name
    CKM
  • Target description
    creatine kinase, M-type
  • Target synonyms
    CKMM; CPK-M; creatine kinase M chain; creatine kinase M-type; creatine kinase, muscle; creatine phosphokinase M-type; M-CK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06732
  • Protein Name
    Creatine kinase M-type
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded rat skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Creatine Kinase MM Polyclonal Antibody, Unconjugated (bs-7530R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product group Antibodies
References
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Figure 1. Western blot analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysate, Lane 2: human CNE whole cell lysate, Lane 3: human COLO-320 whole cell lysate, Lane 4: rat heart tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Creatine Kinase MM antigen affinity purified polyclonal antibody (Catalog # A03452-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Creatine Kinase MM at approximately 43KD. The expected band size for Creatine Kinase MM is at 43KD.
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