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ProductsBack/Creatine kinase (muscle) antibody [N1C3]

Creatine kinase (muscle) antibody [N1C3]

GTX103792
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCKM
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Overview

  • Supplier
    GeneTex
  • Product Name
    Creatine kinase (muscle) antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.71 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1158
  • Target name
    CKM
  • Target description
    creatine kinase, M-type
  • Target synonyms
    CKMM; CPK-M; creatine kinase M chain; creatine kinase M-type; creatine kinase, muscle; creatine phosphokinase M-type; M-CK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06732
  • Protein Name
    Creatine kinase M-type
  • Scientific Description
    The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysate, Lane 2: human CNE whole cell lysate, Lane 3: human COLO-320 whole cell lysate, Lane 4: rat heart tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Creatine Kinase MM antigen affinity purified polyclonal antibody (Catalog # A03452-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Creatine Kinase MM at approximately 43KD. The expected band size for Creatine Kinase MM is at 43KD.
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