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WB analysis of various sample lysates using GTX32526 Clathrin Heavy chain antibody. Dilution : 1:500 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32526 Clathrin Heavy chain antibody. Dilution : 1:500 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32526 Clathrin Heavy chain antibody. Dilution : 1:500 Loading : 25microg per lane

Clathrin Heavy chain antibody

GTX32526
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCLTC
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Overview

  • Supplier
    GeneTex
  • Product Name
    Clathrin Heavy chain antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1213
  • Target name
    CLTC
  • Target description
    clathrin heavy chain
  • Target synonyms
    CHC; CHC17; clathrin heavy chain 1; clathrin heavy chain on chromosome 17; clathrin, heavy polypeptide (Hc); clathrin, heavy polypeptide-like 2; CLH-17; CLTCL2; Hc; MRD56
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ00610
  • Protein Name
    Clathrin heavy chain 1
  • Scientific Description
    Clathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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IHC-P analysis of human breast carcinoma tissue using GTX22731 Clathrin heavy chain antibody [X22]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
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IHC image of CSB-PA005593LA01HU diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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