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Goat anti-CLTC

EB09305
Everest Biotech
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCLTC
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-CLTC Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence:: This antibody has been successfully used in IF on Human: Marinval N et al. (2016) PMID: 27763505.
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IHC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1213
  • Target name
    CLTC
  • Target description
    clathrin heavy chain
  • Target synonyms
    CHC; CHC17; clathrin heavy chain 1; clathrin heavy chain on chromosome 17; clathrin, heavy polypeptide (Hc); clathrin, heavy polypeptide-like 2; CLH-17; CLTCL2; Hc; MRD56
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004850.1. Purification: Antigen affinity purified. Names and symbols: CLTC; clathrin, heavy chain (Hc); CHC17; CLH-17; CLTCL2; Hc; KIAA0034; clathrin heavy chain 1; clathrin, heavy polypeptide (Hc); clathrin, heavy polypeptide-like 2
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Identification of a Pro-Angiogenic Potential and Cellular Uptake Mechanism of a LMW Highly Sulfated Fraction of Fucoidan from Ascophyllum nodosum. Marinval N et al., 2016 Oct 17, Mar Drugs
    Read more

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Figure 1. Western blot analysis of Clathrin heavy chain/CLTC using anti-Clathrin heavy chain/CLTC antibody (A03134-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Clathrin heavy chain/CLTC antigen affinity purified polyclonal antibody (Catalog # A03134-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Clathrin heavy chain/CLTC at approximately 192KD. The expected band size for Clathrin heavy chain/CLTC is at 192KD.
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