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Untransfected KATO3 cells (green line), transfected Human CLDN18.2 KATO3 stable cells (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells (blue line) were stained with anti-CLDN18.2 antibody (2microg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
Untransfected KATO3 cells (green line), transfected Human CLDN18.2 KATO3 stable cells (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells (blue line) were stained with anti-CLDN18.2 antibody (2microg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
Untransfected KATO3 cells (green line), transfected Human CLDN18.2 KATO3 stable cells (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells (blue line) were stained with anti-CLDN18.2 antibody (2microg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

CLDN18 Recombinant Monoclonal Antibody

CSB-RA005498A2HU
Cusabio
ApplicationsFlow Cytometry, ELISA
Product group Antibodies
ReactivityHuman
TargetCLDN18
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Overview

  • Supplier
    Cusabio
  • Product Name
    CLDN18 Recombinant Monoclonal Antibody
  • Delivery Days Customer
    20
  • Applications
    Flow Cytometry, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    25E3
  • Conjugate
    Unconjugated
  • Gene ID51208
  • Target name
    CLDN18
  • Target description
    claudin 18
  • Target synonyms
    claudin-18; SFTA5; SFTPJ; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J
  • Isotype
    IgG1
  • Protein IDP56856
  • Protein Name
    Claudin-18
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Claudin18/CLDN18 using anti-Claudin18/CLDN18 antibody (A09129-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Claudin18/CLDN18 antigen affinity purified polyclonal antibody (Catalog # A09129-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Claudin18/CLDN18 at approximately 30 kDa. The expected band size for Claudin18/CLDN18 is at 30 kDa.
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