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CLEC9A Antibody

LS-C832660
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCLEC9A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CLEC9A Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:10000), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.08 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID283420
  • Target name
    CLEC9A
  • Target description
    C-type lectin domain containing 9A
  • Target synonyms
    CD370, DNGR-1, DNGR1, UNQ9341, C-type lectin domain family 9 member A, HEEE9341
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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The Binding Activity of Human CLEC9A with Anti-CLEC9A recombinant antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized Anti-CLEC9A recombinant antibody at 2 microg/mL can bind Human CLEC9A protein (CSB-MP740922HU-B). The EC50 is 2.915-3.520 ng/mL.
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Figure 1. Flow Cytometry analysis of Jurkat cells using anti-CLEC9A antibody (A04577). Overlay histogram showing Jurkat cells stained with A04577 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CLEC9A Antibody (A04577, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CLEC9A Polyclonal Antibody, Unconjugated (bs-13620R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
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