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Western blot All lanes: CLGN antibody at 7microg/ml Lane 1: Mouse brain tissue Lane 2: Mouse lung tissue Lane 3: Jurkat whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: PC-3 whole cell lysate Lane 6: Hela whole cell lysate Lane 7: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 47 kDa Observed band size: 80 kDa
Western blot All lanes: CLGN antibody at 7microg/ml Lane 1: Mouse brain tissue Lane 2: Mouse lung tissue Lane 3: Jurkat whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: PC-3 whole cell lysate Lane 6: Hela whole cell lysate Lane 7: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 47 kDa Observed band size: 80 kDa
Western blot All lanes: CLGN antibody at 7microg/ml Lane 1: Mouse brain tissue Lane 2: Mouse lung tissue Lane 3: Jurkat whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: PC-3 whole cell lysate Lane 6: Hela whole cell lysate Lane 7: HL60 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 71, 47 kDa Observed band size: 80 kDa

CLGN Antibody

CSB-PA005544LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCLGN
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Overview

  • Supplier
    Cusabio
  • Product Name
    CLGN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1047
  • Target name
    CLGN
  • Target description
    calmegin
  • Target synonyms
    calmegin; testis tissue sperm-binding protein Li 79P
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14967
  • Protein Name
    Calmegin
  • Scientific Description
    Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Calmegin/CLGN using anti-Calmegin/CLGN antibody (A05261-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calmegin/CLGN antigen affinity purified polyclonal antibody (Catalog # A05261-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calmegin/CLGN at approximately 93 kDa. The expected band size for Calmegin/CLGN is at 93 kDa.
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