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ProductsBack/CLGN antibody, N-term
WB analysis of HepG2 cells using GTX46439 CLGN antibody at 2.5μg/ml.
WB analysis of HepG2 cells using GTX46439 CLGN antibody at 2.5μg/ml.
WB analysis of HepG2 cells using GTX46439 CLGN antibody at 2.5μg/ml.

CLGN antibody, N-term

GTX46439
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetCLGN
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Overview

  • Supplier
    GeneTex
  • Product Name
    CLGN antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1047
  • Target name
    CLGN
  • Target description
    calmegin
  • Target synonyms
    calmegin, testis tissue sperm-binding protein Li 79P
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14967
  • Protein Name
    Calmegin
  • Scientific Description
    Calmegin is a testis-specific endoplasmic reticulum chaperone protein. CLGN may play a role in spermatogeneisis and infertility. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Calmegin/CLGN using anti-Calmegin/CLGN antibody (A05261-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calmegin/CLGN antigen affinity purified polyclonal antibody (Catalog # A05261-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calmegin/CLGN at approximately 93 kDa. The expected band size for Calmegin/CLGN is at 93 kDa.
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