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WB analysis of various sample lysates using GTX65866 CLIC1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX65866 CLIC1 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX65866 CLIC1 antibody. Dilution : 1:1000 Loading : 25microg per lane

CLIC1 antibody

GTX65866
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCLIC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CLIC1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1192
  • Target name
    CLIC1
  • Target description
    chloride intracellular channel 1
  • Target synonyms
    chloride channel ABP; chloride intracellular channel protein 1; CL1C1; CLCNL1; G6; hRNCC; NCC27; nuclear chloride ion channel 27; nuclear chloride ion channel protein; p64CLCP; regulatory nuclear chloride ion channel protein; RNCC protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00299
  • Protein Name
    Chloride intracellular channel protein 1
  • Scientific Description
    Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 1 is a member of the p64 family; the protein localizes principally to the cell nucleus and exhibits both nuclear and plasma membrane chloride ion channel activity. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CLIC1 using anti-CLIC1 antibody (A02397-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: human U-937 whole cell lysates, Lane 7: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIC1 antigen affinity purified polyclonal antibody (Catalog # A02397-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLIC1 at approximately 32 kDa. The expected band size for CLIC1 is at 32 kDa.
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