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ProductsBack/CLPB Antibody - middle region (ARP53791_P050)

CLPB Antibody - middle region (ARP53791_P050)

ORB330730
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Rabbit, Rat
TargetCLPB
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Overview

  • Supplier
    Biorbyt
  • Product Name
    CLPB Antibody - middle region (ARP53791_P050)
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID81570
  • Target name
    CLPB
  • Target description
    ClpB family mitochondrial disaggregase
  • Target synonyms
    ANKCLB, ANKCLP, HSP78, MEGCANN, MGCA7, MGCA7A, SCN9, SKD3, mitochondrial disaggregase, ClpB caseinolytic peptidase B homolog, ClpB homolog, mitochondrial AAA ATPase chaperonin, ankyrin-repeat containing bacterial clp fusion, caseinolytic mitochondrial matrix peptidase chaperone subunit B, caseinolytic peptidase B protein homolog, suppressor of potassium transport defect 3, testicular secretory protein Li 11
  • Host
    Rabbit
  • Protein IDQ9H078
  • Protein Name
    Mitochondrial disaggregase
  • Scientific Description
    Rabbit polyclonal antibody to CLPB
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CLPB using anti-CLPB antibody (A02912-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: huamn SW620 whole cell lysates, Lane 3: huamn 22RV1 whole cell lysates, Lane 4: huamn THP-1 whole cell lysates, Lane 5: human SGC-7901 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse heart tissue lysates, Lane 9: mouse HEPA1-6 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLPB antigen affinity purified polyclonal antibody (Catalog # A02912-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLPB at approximately 70KD. The expected band size for CLPB is at 70KD.
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