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CNN3 Polyclonal Antibody

CAC14714
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetCNN3
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Overview

  • Supplier
    Biomatik
  • Product Name
    CNN3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1266
  • Target name
    CNN3
  • Target description
    calponin 3
  • Target synonyms
    calponin-3, calponin 3, acidic, dJ639P13.2.2 (acidic calponin 3)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15417
  • Protein Name
    Calponin-3
  • Scientific Description
    The CNN3 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Calponin 3/CNN3 using anti-Calponin 3/CNN3 antibody (A08267-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calponin 3/CNN3 antigen affinity purified polyclonal antibody (Catalog # A08267-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calponin 3/CNN3 at approximately 36 kDa. The expected band size for Calponin 3/CNN3 is at 36 kDa.
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