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ProductsBack/Goat anti-Calponin 3 / CNN3 Antibody

Goat anti-Calponin 3 / CNN3 Antibody

EB09526
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCNN3
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Calponin 3 / CNN3 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1266
  • Target name
    CNN3
  • Target description
    calponin 3
  • Target synonyms
    calponin-3, calponin 3, acidic, dJ639P13.2.2 (acidic calponin 3)
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001830.1. Purification: Antigen affinity purified. Names and symbols: CNN3; calponin 3, acidic; calponin 3; calponin, acidic; dJ639P13.2.2 (acidic calponin 3)
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Calponin 3/CNN3 using anti-Calponin 3/CNN3 antibody (A08267-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calponin 3/CNN3 antigen affinity purified polyclonal antibody (Catalog # A08267-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calponin 3/CNN3 at approximately 36 kDa. The expected band size for Calponin 3/CNN3 is at 36 kDa.
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