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Western Blot analysis of HuvEc cells using CNOT7 Polyclonal Antibody
Western Blot analysis of HuvEc cells using CNOT7 Polyclonal Antibody
Western Blot analysis of HuvEc cells using CNOT7 Polyclonal Antibody

CNOT7 Antibody

CSB-PA001734
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCNOT7
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Overview

  • Supplier
    Cusabio
  • Product Name
    CNOT7 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29883
  • Target name
    CNOT7
  • Target description
    CCR4-NOT transcription complex subunit 7
  • Target synonyms
    CAF-1, CAF1, Caf1a, hCAF-1, CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1, carbon catabolite repressor protein (CCR4)-associative factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UIV1
  • Protein Name
    CCR4-NOT transcription complex subunit 7
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CNOT7 using anti-CNOT7 antibody (A04884-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNOT7 antigen affinity purified polyclonal antibody (Catalog # A04884-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNOT7 at approximately 33 kDa. The expected band size for CNOT7 is at 33 kDa.
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