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CNOT7 Antibody

LS-C832457
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCNOT7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CNOT7 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:50 - 1:100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.02 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID29883
  • Target name
    CNOT7
  • Target description
    CCR4-NOT transcription complex subunit 7
  • Target synonyms
    CAF-1, CAF1, Caf1a, hCAF-1, CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1, carbon catabolite repressor protein (CCR4)-associative factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CNOT7 using anti-CNOT7 antibody (A04884-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human RT4 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CNOT7 antigen affinity purified polyclonal antibody (Catalog # A04884-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CNOT7 at approximately 33 kDa. The expected band size for CNOT7 is at 33 kDa.
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