Bio-Connect

CNTF antibody [AS23]

GTX17284
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
TargetCNTF
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    CNTF antibody [AS23]
  • Delivery Days Customer
    9
  • Application Supplier Note
    ELISA: This antibody has been used as a coating antibody at 10 ug/ml in a two site ELISA with PS2-B. Typically the ELISA has a working range of 0-20000 pg/ml and a sensitivity of 10 pg/ml when used with biotin conjugated CNTF antibody. Recommended sample volumes are 25-200 microl. Recovery of hCNTF in human plasma is typically > 85 in the ELISA. IP: This antibody has been used to immunoprecipitate >90% hCNTF in solution using a double-antibody method. WB: Use at a concentration of 1 - 5 microg/ml. Predicted molecular weight: 24 kDa. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AS23
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1270
  • Target name
    CNTF
  • Target description
    ciliary neurotrophic factor
  • Target synonyms
    Ciliary Neuronotrophic Factor; ciliary neurotrophic factor; HCNTF
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP26441
  • Protein Name
    Ciliary neurotrophic factor
  • Scientific Description
    The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Formalin-fixed and paraffin embedded rat brain labeled with Anti-CNTF Polyclonal Antibody, Unconjugated (bs-1272R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Product group Antibodies
References
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetCNTF
  • SizePrice
Product group Antibodies
Assay Genie
TargetCNTF
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCNTF
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetCNTF
  • SizePrice
Anti-CNTF Antibody
Product group Antibodies
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • SizePrice
Product group Antibodies
Biosynth
CNTF antibody70R-CR006
  • SizePrice
Immunofluorescence staining of U251 cells with CSB-PA06039A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product group Antibodies
Cusabio
CNTF AntibodyCSB-PA06039A0RB
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetCNTF
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CNTF AntibodyLS-C763473
ApplicationsImmunoHistoChemistry
TargetCNTF
  • SizePrice
Immunohistochemical staining of human Cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCNTF
  • SizePrice
Figure 1. IHC analysis of CNTF using anti-CNTF antibody (PB9012). CNTF was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-CNTF Antibody (PB9012) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetCNTF
  • SizePrice