Immunoprecipitation of Coilin protein from 293T whole cell extracts using 5 μg of Coilin antibody (GTX112570). Western blot analysis was performed using Coilin antibody (GTX112570). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunoprecipitation of Coilin protein from 293T whole cell extracts using 5 μg of Coilin antibody (GTX112570). Western blot analysis was performed using Coilin antibody (GTX112570). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Coilin antibody
GTX112570
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCOIL
Overview
- SupplierGeneTex
- Product NameCoilin antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.66 mg/ml
- ConjugateUnconjugated
- Gene ID8161
- Target nameCOIL
- Target descriptioncoilin
- Target synonymsCLN80, p80-coilin, coilin, coilin p80, p80
- HostRabbit
- IsotypeIgG
- Protein IDP38432
- Protein NameCoilin
- Scientific DescriptionThe protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Rivera C, Verbel-Vergara D, Arancibia D, et al. Revealing RCOR2 as a regulatory component of nuclear speckles. Epigenetics Chromatin. 2021,14(1):51. doi: 10.1186/s13072-021-00425-4Read this paper
- Bogard B, Francastel C, Hubé F. Systematic Identification and Functional Validation of New snoRNAs in Human Muscle Progenitors. Noncoding RNA. 2021,7(3). doi: 10.3390/ncrna7030056Read this paper
- Sandhu R, Sharma M, Wei D, et al. The structurally conserved TELR region on shelterin protein TPP1 is essential for telomerase processivity but not recruitment. Proc Natl Acad Sci U S A. 2021,118(30). doi: 10.1073/pnas.2024889118Read this paper
Datasheet
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