HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Coilin antibody [HL2384] (GTX638593) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Coilin antibody [HL2384] detects Coilin protein at Cajal bodies by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Coilin stained by Coilin antibody [HL2384] (GTX638593) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX638593/GTX638593_45131_20240607_ICC_IF_24070822_852.webp "Coilin antibody [HL2384] detects Coilin protein at Cajal bodies by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Coilin stained by Coilin antibody [HL2384] (GTX638593) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Coilin antibody [HL2384] (GTX638593) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Coilin antibody [HL2384]
GTX638593
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetCOIL
Overview
- SupplierGeneTex
- Product NameCoilin antibody [HL2384]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2384
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8161
- Target nameCOIL
- Target descriptioncoilin
- Target synonymsCLN80, p80-coilin, coilin, coilin p80, p80
- HostRabbit
- IsotypeIgG
- Protein IDP38432
- Protein NameCoilin
- Scientific DescriptionThe protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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