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Col17A1 Polyclonal Antibody

CAC10429
Biomatik
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCOL17A1
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Overview

  • Supplier
    Biomatik
  • Product Name
    COL17A1 Antibody
  • Delivery Days Customer
    12
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC; Recommended dilution: IHC:1:10-1:100
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1308
  • Target name
    COL17A1
  • Target description
    collagen type XVII alpha 1 chain
  • Target synonyms
    180 kDa bullous pemphigoid antigen 2; alpha 1 type XVII collagen; BA16H23.2; bA16H23.2 (collagen, type XVII, alpha 1 (BP180)); BP180; BPA-2; BPAG2; bullous pemphigoid antigen 2 (180kD); collagen alpha-1(XVII) chain; collagen XVII, alpha-1 polypeptide; collagen, type XVII, alpha 1; ERED; LAD-1; type XVII collagen alpha-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UMD9
  • Protein Name
    Collagen alpha-1(XVII) chain
  • Scientific Description
    The Col17A1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COL17A1 using anti-COL17A1 antibody (A03031-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL17A1 antigen affinity purified polyclonal antibody (Catalog # A03031-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL17A1 at approximately 125KD. The expected band size for COL17A1 is at 150KD.
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