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ProductsBack/Collagen XVII Recombinant Antibody
Paraformaldehyde-fixed, paraffin embedded mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Collagen XVII (1C11) Monoclonal Antibody (bsm-52041R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Collagen XVII (1C11) Monoclonal Antibody (bsm-52041R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse skin; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer at 37°C for 20min; Antibody incubation with Collagen XVII (1C11) Monoclonal Antibody (bsm-52041R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Collagen XVII Recombinant Antibody

BSM-52041R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCOL17A1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Collagen 17 (1C11) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID1308
  • Target name
    COL17A1
  • Target description
    collagen type XVII alpha 1 chain
  • Target synonyms
    180 kDa bullous pemphigoid antigen 2; alpha 1 type XVII collagen; BA16H23.2; bA16H23.2 (collagen, type XVII, alpha 1 (BP180)); BP180; BPA-2; BPAG2; bullous pemphigoid antigen 2 (180kD); collagen alpha-1(XVII) chain; collagen XVII, alpha-1 polypeptide; collagen, type XVII, alpha 1; ERED; LAD-1; type XVII collagen alpha-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UMD9
  • Protein Name
    Collagen alpha-1(XVII) chain
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of COL17A1 using anti-COL17A1 antibody (A03031-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL17A1 antigen affinity purified polyclonal antibody (Catalog # A03031-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL17A1 at approximately 125KD. The expected band size for COL17A1 is at 150KD.
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