COL6A1 antibody [C1C2], Internal detects COL6A1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. COL6A1 antibody [C1C2], Internal (GTX109963) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![Wild-type (WT) and COL6A1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COL6A1 antibody [C1C2], Internal (GTX109963) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109963/GTX109963_40002_20171215_WB_KO_watermark_w_23060500_641.webp "Wild-type (WT) and COL6A1 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COL6A1 antibody [C1C2], Internal (GTX109963) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![COL6A1 antibody [C1C2], Internal detects COL6A1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. COL6A1 antibody [C1C2], Internal (GTX109963) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109963/GTX109963_40002_IHC_w_23060500_352.webp "COL6A1 antibody [C1C2], Internal detects COL6A1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. COL6A1 antibody [C1C2], Internal (GTX109963) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![COL6A1 antibody [C1C2], Internal detects COL6A1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COL6A1 stained by COL6A1 antibody [C1C2], Internal (GTX109963) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX109963/GTX109963_40002_20181227_ICC_IF_R_w_23060500_953.webp "COL6A1 antibody [C1C2], Internal detects COL6A1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COL6A1 stained by COL6A1 antibody [C1C2], Internal (GTX109963) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with COL6A1 antibody [C1C2], Internal (GTX109963) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109963/GTX109963_40002_20170518_WB_w_23060500_181.webp "Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with COL6A1 antibody [C1C2], Internal (GTX109963) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
COL6A1 antibody [C1C2], Internal detects COL6A1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. COL6A1 antibody [C1C2], Internal (GTX109963) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
COL6A1 antibody [C1C2], Internal
GTX109963
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCOL6A1
Overview
- SupplierGeneTex
- Product NameCOL6A1 antibody [C1C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1291
- Target nameCOL6A1
- Target descriptioncollagen type VI alpha 1 chain
- Target synonymsBTHLM1, BTHLM1A, OPLL, UCHMD1, UCHMD1A, collagen alpha-1(VI) chain, alpha 1 (VI) chain (61 AA), collagen VI, alpha-1 polypeptide, collagen, type VI, alpha 1, epididymis secretory sperm binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP12109
- Protein NameCollagen alpha-1(VI) chain
- Scientific DescriptionThe collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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