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ProductsBack/Collagen VI alpha 1 Polyclonal Antibody
NIH3T3 lysates probed with Collagen 6 alpha 1 Polyclonal Antibody, Unconjugated (bs-0553R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
NIH3T3 lysates probed with Collagen 6 alpha 1 Polyclonal Antibody, Unconjugated (bs-0553R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
NIH3T3 lysates probed with Collagen 6 alpha 1 Polyclonal Antibody, Unconjugated (bs-0553R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Collagen VI alpha 1 Polyclonal Antibody

BS-0553R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCOL6A1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Collagen 6 alpha 1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1291
  • Target name
    COL6A1
  • Target description
    collagen type VI alpha 1 chain
  • Target synonyms
    alpha 1 (VI) chain (61 AA); BTHLM1; collagen alpha-1(VI) chain; collagen VI, alpha-1 polypeptide; collagen, type VI, alpha 1; epididymis secretory sperm binding protein; OPLL; UCHMD1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12109
  • Protein Name
    Collagen alpha-1(VI) chain
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Alteration and prognostic values of collagen gene expression in patients with gastric cancer under different treatments. Gao X et al., 2020 Mar, Pathol Res Pract
    Read more

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Figure 1. Western blot analysis of COL6A1 using anti-COL6A1 antibody (A02226-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL6A1 antigen affinity purified polyclonal antibody (Catalog # A02226-5) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL6A1 at approximately 140 kDa. The expected band size for COL6A1 is at 109 kDa.
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