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Western blot analysis of extracts of various cell lines using Collagen VI/Collagen VI/COL6A1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using Collagen VI/Collagen VI/COL6A1 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using Collagen VI/Collagen VI/COL6A1 Polyclonal Antibody at 1:1000 dilution.

COL6A1 Polyclonal Antibody

E-AB-63945
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    COL6A1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The alpha2(VI) and alpha3(VI) chains are encoded by the COL6A2 and COL6A3 genes, respectively. The protein encoded by this gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COL6A1 using anti-COL6A1 antibody (A02226-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL6A1 antigen affinity purified polyclonal antibody (Catalog # A02226-5) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL6A1 at approximately 140 kDa. The expected band size for COL6A1 is at 109 kDa.
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NIH3T3 lysates probed with Collagen 6 alpha 1 Polyclonal Antibody, Unconjugated (bs-0553R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Product group Antibodies
References
Bioss
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Immunofluorescence staining of Hela cells with CSB-PA005751NA01HU at 1:180, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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