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Collagen VII Polyclonal Antibody

BS-1558R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetCOL7A1
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Overview

  • Supplier
    Bioss
  • Product Name
    Collagen VII Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    ELISA(1:500-1000), IHC-F(1:100-500), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1294
  • Target name
    COL7A1
  • Target description
    collagen type VII alpha 1 chain
  • Target synonyms
    EBD1, EBDCT, EBR1, NDNC8, collagen alpha-1(VII) chain, LC collagen, collagen VII, alpha-1 polypeptide, collagen, type VII, alpha 1, long-chain collagen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02388
  • Protein Name
    Collagen alpha-1(VII) chain
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Torkamani N, Rufaut NW, Jones L, et al. Epidermal Cells Expressing Putative Cell Markers in Nonglabrous Skin Existing in Direct Proximity with the Distal End of the Arrector Pili Muscle. Stem Cells Int. 2016,2016:1286315. doi: 10.1155/2016/1286315
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Collagen VII/COL7A1 using anti-Collagen VII/COL7A1 antibody (A01170-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen VII/COL7A1 antigen affinity purified polyclonal antibody (Catalog # A01170-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen VII/COL7A1 at approximately 300KD. The expected band size for Collagen VII/COL7A1 is at 300KD.
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