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ProductsBack/Goat anti-Collagen, type VII

Goat anti-Collagen, type VII

EB06781
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCOL7A1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Collagen, type VII Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    A customer found this antibody unsuccessful on cryosections of Human Skin.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1294
  • Target name
    COL7A1
  • Target description
    collagen type VII alpha 1 chain
  • Target synonyms
    EBD1, EBDCT, EBR1, NDNC8, collagen alpha-1(VII) chain, LC collagen, collagen VII, alpha-1 polypeptide, collagen, type VII, alpha 1, long-chain collagen
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000085.1. GeneIDs all Nonhuman: 12836 (mouse);. Purification: Antigen affinity purified. Names and symbols: Collagen, type VII; collagen, type VII, alpha 1 (epidermolysis bullosa, dystrophic, dominant and recessive); COL7A1; HGNC:2214; EBD1; EBDCT; EBR1; LC collagen; alpha 1 type VII collagen; collagen VII; alpha-1 polypeptide; long chain collagen; collagen, ty
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of Collagen VII/COL7A1 using anti-Collagen VII/COL7A1 antibody (A01170-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen VII/COL7A1 antigen affinity purified polyclonal antibody (Catalog # A01170-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen VII/COL7A1 at approximately 300KD. The expected band size for Collagen VII/COL7A1 is at 300KD.
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