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WB analysis of human colon tissue lysate using GTX17229 Connexin 26 antibody. Working concentration : (A) 1 and (B) 2 microg/ml
WB analysis of human colon tissue lysate using GTX17229 Connexin 26 antibody. Working concentration : (A) 1 and (B) 2 microg/ml
WB analysis of human colon tissue lysate using GTX17229 Connexin 26 antibody. Working concentration : (A) 1 and (B) 2 microg/ml

Connexin 26 antibody

GTX17229
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGJB2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Connexin 26 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2706
  • Target name
    GJB2
  • Target description
    gap junction protein beta 2
  • Target synonyms
    BAPS; connexin 26; CX26; DFNA3; DFNA3A; DFNB1; DFNB1A; gap junction beta 2 proteinc; gap junction beta-2 protein; gap junction protein, beta 2, 26kDa; HID; KID; mutant gap junction beta 2 protein; mutant gap junction protein beta 2; NSRD1; PPK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29033
  • Protein Name
    Gap junction beta-2 protein
  • Scientific Description
    This gene encodes a member of the gap junction protein family. The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene are responsible for as much as 50% of pre-lingual, recessive deafness. [provided by RefSeq, Oct 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GJB2 using anti-GJB2 antibody (A00512-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJB2 antigen affinity purified polyclonal antibody (Catalog # A00512-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJB2 at approximately 26 kDa. The expected band size for GJB2 is at 26 kDa.
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