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ProductsBack/Goat anti-GJB2 / Connexin 26

Goat anti-GJB2 / Connexin 26

EB07536
Everest Biotech
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetGJB2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-GJB2 / Connexin 26 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2706
  • Target name
    GJB2
  • Target description
    gap junction protein beta 2
  • Target synonyms
    BAPS; connexin 26; CX26; DFNA3; DFNA3A; DFNB1; DFNB1A; gap junction beta 2 proteinc; gap junction beta-2 protein; gap junction protein, beta 2, 26kDa; HID; KID; mutant gap junction beta 2 protein; mutant gap junction protein beta 2; NSRD1; PPK
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003995.2. GeneIDs all Nonhuman: 14619 (mouse); 394266 (rat);. Purification: Antigen affinity purified. Names and symbols: GJB2; Connexin 26; gap junction protein, beta 2, 26kDa (connexin 26) ; CX26; DFNA3; DFNB1; HID; KID; NSRD1; PPK ; gap junction protein beta 2; gap junction protein, beta 2, 26kD (connexin 26)
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GJB2 using anti-GJB2 antibody (A00512-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJB2 antigen affinity purified polyclonal antibody (Catalog # A00512-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJB2 at approximately 26 kDa. The expected band size for GJB2 is at 26 kDa.
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