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Connexin 43 Antibody(Rabbit)

HY-P80628
MedChem Express
TargetGJA1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Connexin 43 Antibody(Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID2697
  • Target name
    GJA1
  • Target description
    gap junction protein alpha 1
  • Target synonyms
    AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA, gap junction alpha-1 protein, connexin-43, gap junction 43 kDa heart protein, gap junction protein, alpha 1, 43kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Connexin 43 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Connexin 43.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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MCF-7 cells were stained with Connexin 43 Polyclonal Antibody, Unconjugated(bs-0651R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
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Figure 1. Western blot analysis of GJA1 using anti-GJA1 antibody (A00599). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse testicular tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA1 antigen affinity purified polyclonal antibody (Catalog # A00599) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJA1 at approximately 43KD. The expected band size for GJA1 is at 43KD.
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