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Connexin 47 Polyclonal Antibody

RD76220A
Reddot Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGJC2
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    Connexin 47 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID57165
  • Target name
    GJC2
  • Target description
    gap junction protein gamma 2
  • Target synonyms
    CX46.6, Cx47, GJA12, HLD2, LMPH1C, LMPHM3, PMLDAR, SPG44, gap junction gamma-2 protein, connexin-46.6, connexin-47, gap junction alpha-12 protein, gap junction protein, gamma 2, 47kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a gap junction protein. Gap junction proteins are members of a large family of homologous connexins and comprise 4 transmembrane, 2 extracellular, and 3 cytoplasmic domains. This gene plays a key role in central myelination and is involved in peripheral myelination in humans. Defects in this gene are the cause of autosomal recessive Pelizaeus-Merzbacher-like disease-1.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GJC2 using anti-GJC2 antibody (PB9706). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U20S Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJC2 antigen affinity purified polyclonal antibody (Catalog # PB9706) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJC2 at approximately 47 kDa. The expected band size for GJC2 is at 47 kDa.
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