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WB analysis of various sample lysates using GTX64942 COPE antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64942 COPE antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64942 COPE antibody. Dilution : 1:1000 Loading : 25microg per lane

COPE antibody

GTX64942
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetCOPE
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Overview

  • Supplier
    GeneTex
  • Product Name
    COPE antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11316
  • Target name
    COPE
  • Target description
    COPI coat complex subunit epsilon
  • Target synonyms
    coatomer epsilon subunit; coatomer protein complex subunit epsilon; coatomer subunit epsilon; epsilon coat protein; epsilon-COP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14579
  • Protein Name
    Coatomer subunit epsilon
  • Scientific Description
    The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta, gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COPE using anti-COPE antibody (A04544). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat stomach tissue lysate,Lane 2: rat small intestine tissue lysate,Lane 3: rat pancreas tissue lysate,Lane 4: mouse stomach tissue lysate,Lane 5: mouse small intestine tissue lysate,Lane 6: mouse pancreas tissue lysate,Lane 7: human MCF-7 whole Cell lysatem,Lane 8: human Hela whole Cell lysate,Lane 9: human 22RV1 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COPE antigen affinity purified polyclonal antibody (Catalog # A04544) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COPE at approximately 34KD. The expected band size for COPE is at 34KD.
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