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COX1/PTGS1 Monoclonal Antibody

CAB4301
Assay Genie
TargetPTGS1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    COX1/PTGS1 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID5742
  • Target name
    PTGS1
  • Target description
    prostaglandin-endoperoxide synthase 1
  • Target synonyms
    COX1; COX3; cyclooxygenase-1; PCOX1; PES-1; PGG/HS; PGH synthase 1; PGHS1; PGHS-1; PHS1; prostaglandin G/H synthase 1; prostaglandin H2 synthase 1; prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase); PTGHS
  • Host
    Rabbit
  • Protein IDP23219
  • Protein Name
    Prostaglandin G/H synthase 1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat L6 whole cell lysates, Lane 4: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX1/Cyclooxygenase 1/PTGS1 antigen affinity purified polyclonal antibody (Catalog # PB9002) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX1/Cyclooxygenase 1/PTGS1 at approximately 65-72 kDa. The expected band size for COX1/Cyclooxygenase 1/PTGS1 is at 69 kDa.
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