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ProductsBack/Cyclooxygenase 1 Polyclonal Antibody
Lane 1: NIH/3T3 lysates; Lane 2: Hela lysates; Lane 3: Huvec lysates probed with Cyclooxygenase 1 Polyclonal Antibody, Unconjugated (bs-0582R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: NIH/3T3 lysates; Lane 2: Hela lysates; Lane 3: Huvec lysates probed with Cyclooxygenase 1 Polyclonal Antibody, Unconjugated (bs-0582R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: NIH/3T3 lysates; Lane 2: Hela lysates; Lane 3: Huvec lysates probed with Cyclooxygenase 1 Polyclonal Antibody, Unconjugated (bs-0582R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Cyclooxygenase 1 Polyclonal Antibody

BS-0582R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
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Overview

  • Supplier
    Bioss
  • Product Name
    Cyclooxygenase 1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ63921
  • Protein Name
    Prostaglandin G/H synthase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wang Y, Yao Y, Zhang C, et al. Seasonal expressions of COX-1, COX-2 and EP4 in the uteri of the wild Daurian ground squirrels (Spermophilus dauricus). Prostaglandins Other Lipid Mediat. 2019,143:106343. doi: 10.1016/j.prostaglandins.2019.106343
    Read this paper
  • Hu R, Xi L, Cao Q, et al. The expression of prostaglandin-E2 and its receptor in the oviduct of Chinese brown frog (Rana dybowskii). Prostaglandins Other Lipid Mediat. 2016,124:9-15. doi: 10.1016/j.prostaglandins.2016.05.006
    Read this paper
  • Li W, Wang J, Jiang HR, et al. Combined effects of cyclooxygenase-1 and cyclooxygenase-2 selective inhibitors on ovarian carcinoma in vivo. Int J Mol Sci. 2011,12(1):668-81. doi: 10.3390/ijms12010668
    Read this paper

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Figure 1. Western blot analysis of COX1/Cyclooxygenase 1/PTGS1 using anti-COX1/Cyclooxygenase 1/PTGS1 antibody (PB9002). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat L6 whole cell lysates, Lane 4: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COX1/Cyclooxygenase 1/PTGS1 antigen affinity purified polyclonal antibody (Catalog # PB9002) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COX1/Cyclooxygenase 1/PTGS1 at approximately 65-72 kDa. The expected band size for COX1/Cyclooxygenase 1/PTGS1 is at 69 kDa.
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