![WB analysis of HEK293 (1), A549 (2) and PC12 (3) cell lysate using GTX82782 COX4 antibody [6B3]. WB analysis of HEK293 (1), A549 (2) and PC12 (3) cell lysate using GTX82782 COX4 antibody [6B3].](https://www.genetex.com/upload/website/prouct_img/normal/GTX82782/GTX82782_20170912_WB_w_23061322_969.webp)
WB analysis of HEK293 (1), A549 (2) and PC12 (3) cell lysate using GTX82782 COX4 antibody [6B3].
COX4 antibody [6B3]
GTX82782
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetCOX4I1
Overview
- SupplierGeneTex
- Product NameCOX4 antibody [6B3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID6B3
- ConjugateUnconjugated
- Gene ID1327
- Target nameCOX4I1
- Target descriptioncytochrome c oxidase subunit 4I1
- Target synonymsCOX IV-1, COX4, COX4-1, COXIV, COXIV-1, MC4DN16, cytochrome c oxidase subunit 4 isoform 1, mitochondrial, cytochrome c oxidase polypeptide IV, cytochrome c oxidase subunit IV
- HostMouse
- IsotypeIgG1
- Protein IDP13073
- Protein NameCytochrome c oxidase subunit 4 isoform 1, mitochondrial
- Scientific DescriptionCytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3 of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Monkey, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![FACS analysis of K562 cells using GTX82782 COX4 antibody [6B3]. Blue : COX4 Red : negative control FACS analysis of K562 cells using GTX82782 COX4 antibody [6B3]. Blue : COX4 Red : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX82782/GTX82782_20170912_FACS_w_23061322_816.webp)
![ICC/IF analysis of PANC-1 cells using GTX82782 COX4 antibody [6B3]. Green : COX4 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments ICC/IF analysis of PANC-1 cells using GTX82782 COX4 antibody [6B3]. Green : COX4 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX82782/GTX82782_20170912_ICCIF_w_23061322_761.webp)


![Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628886/GTX628886_44503_20211112_WB_22110201_302.webp)

![COX4 antibody [HL2264] detects COX4 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. COX4 stained by COX4 antibody [HL2264] (GTX638316) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638316/GTX638316_T-44970_20230324_IHC-P_multiple_M_23032819_438.webp)


