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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: CPSF6 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 64, 53 kDa Observed band size: 60 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: CPSF6 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 64, 53 kDa Observed band size: 60 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: CPSF6 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 64, 53 kDa Observed band size: 60 kDa

CPSF6 Antibody

CSB-PA621966LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCPSF6
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Overview

  • Supplier
    Cusabio
  • Product Name
    CPSF6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11052
  • Target name
    CPSF6
  • Target description
    cleavage and polyadenylation specific factor 6
  • Target synonyms
    CFIM; CFIM68; CFIM72; cleavage and polyadenylation specific factor 6, 68kDa; cleavage and polyadenylation specificity factor 68 kDa subunit; cleavage and polyadenylation specificity factor subunit 6; cleavage factor Im complex 68 kDa subunit; CPSF 68 kDa subunit; HPBRII-4; HPBRII-7; pre-mRNA cleavage factor I, 68kD subunit; pre-mRNA cleavage factor Im (68kD); pre-mRNA cleavage factor Im 68 kDa subunit; protein HPBRII-4/7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16630
  • Protein Name
    Cleavage and polyadenylation specificity factor subunit 6
  • Scientific Description
    Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CPSF6 using anti-CPSF6 antibody (A04551-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPSF6 antigen affinity purified polyclonal antibody (Catalog # A04551-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPSF6 at approximately 68 kDa. The expected band size for CPSF6 is at 68 kDa.
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