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ProductsBack/CPSF6 Antibody
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: CPSF6 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 64, 53 kDa Observed band size: 60 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: CPSF6 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 64, 53 kDa Observed band size: 60 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: CPSF6 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 64, 53 kDa Observed band size: 60 kDa

CPSF6 Antibody

CSB-PA621966LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCPSF6
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Overview

  • Supplier
    Cusabio
  • Product Name
    CPSF6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11052
  • Target name
    CPSF6
  • Target description
    cleavage and polyadenylation specific factor 6
  • Target synonyms
    CFIM, CFIM68, CFIM72, HPBRII-4, HPBRII-7, cleavage and polyadenylation specificity factor subunit 6, CPSF 68 kDa subunit, cleavage and polyadenylation specific factor 6, 68kDa, cleavage and polyadenylation specificity factor 68 kDa subunit, cleavage factor Im complex 68 kDa subunit, pre-mRNA cleavage factor I, 68kD subunit, pre-mRNA cleavage factor Im (68kD), pre-mRNA cleavage factor Im 68 kDa subunit, protein HPBRII-4/7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16630
  • Protein Name
    Cleavage and polyadenylation specificity factor subunit 6
  • Scientific Description
    Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3-processing. Involved in association with NUDT21/CPSF5 in pre-MRNA 3-end poly(A) site cleavage and poly(A) addition. CPSF6 binds to cleavage and polyadenylation RNA substrates and promotes RNA looping.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CPSF6 using anti-CPSF6 antibody (A04551-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPSF6 antigen affinity purified polyclonal antibody (Catalog # A04551-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPSF6 at approximately 68 kDa. The expected band size for CPSF6 is at 68 kDa.
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