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CPT1A antibody [C1C2], Internal

GTX114337
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCPT1A
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Overview

  • Supplier
    GeneTex
  • Product Name
    CPT1A antibody [C1C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1374
  • Target name
    CPT1A
  • Target description
    carnitine palmitoyltransferase 1A
  • Target synonyms
    carnitine O-palmitoyltransferase 1, liver isoform; carnitine palmitoyltransferase 1A (liver); carnitine palmitoyltransferase I, liver; CPT I; CPT1; CPT1-L; CPTI-L; L-CPT1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50416
  • Protein Name
    Carnitine O-palmitoyltransferase 1, liver isoform
  • Scientific Description
    The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Mitochondrial morphology controls fatty acid utilization by changing CPT1 sensitivity to malonyl-CoA.
    Read more
  • Quantitative Profiling of Oxylipin Reveals the Mechanism of Pien-Tze-Huang on Alcoholic Liver Disease. Zhu Z et al., 2021, Evid Based Complement Alternat Med
    Read more
  • Downregulation of fatty acid oxidation by involvement of HIF-1alpha and PPARgamma in human gastric adenocarcinoma and related clinical significance. Ezzeddini R et al., 2021 May, J Physiol Biochem
    Read more
  • Ugonin J improves metabolic disorder and ameliorates nonalcoholic fatty liver disease by regulating the AMPK/AKT signaling pathway. Chang TC et al., 2021 Jan, Pharmacol Res
    Read more
  • Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice. Tsai YT et al., 2020 Mar 10, Nutrients
    Read more
  • Helminthostachys zeylanica alleviates hepatic steatosis and insulin resistance in diet-induced obese mice. Chang TC et al., 2019 Dec 13, BMC Complement Altern Med
    Read more
  • Fenofibrate induces human hepatoma Hep3B cells apoptosis and necroptosis through inhibition of thioesterase domain of fatty acid synthase. You BJ et al., 2019 Mar 1, Sci Rep
    Read more
  • Orlistat Displays Antitumor Activity and Enhances the Efficacy of Paclitaxel in Human Hepatoma Hep3B Cells. You BJ et al., 2019 Feb 18, Chem Res Toxicol
    Read more
  • The nuclear receptor FXR uncouples the actions of miR-33 from SREBP-2. Tarling EJ et al., 2015 Apr, Arterioscler Thromb Vasc Biol
    Read more
  • Loss of HSulf-1 promotes altered lipid metabolism in ovarian cancer. Roy D et al., 2014, Cancer Metab
    Read more

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Figure 1. Western blot analysis of CPT1A using anti-CPT1A antibody (A00917-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT-4 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPT1A antigen affinity purified polyclonal antibody (Catalog # A00917-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPT1A at approximately 88 kDa. The expected band size for CPT1A is at 88 kDa.
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