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Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse kidney tissue All lanes: CPT1A antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 89, 87 kDa Observed band size: 89 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse kidney tissue All lanes: CPT1A antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 89, 87 kDa Observed band size: 89 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Mouse kidney tissue All lanes: CPT1A antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 89, 87 kDa Observed band size: 89 kDa

CPT1A Antibody

CSB-PA005922LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCPT1A
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Overview

  • Supplier
    Cusabio
  • Product Name
    CPT1A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1374
  • Target name
    CPT1A
  • Target description
    carnitine palmitoyltransferase 1A
  • Target synonyms
    carnitine O-palmitoyltransferase 1, liver isoform; carnitine palmitoyltransferase 1A (liver); carnitine palmitoyltransferase I, liver; CPT I; CPT1; CPT1-L; CPTI-L; L-CPT1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50416
  • Protein Name
    Carnitine O-palmitoyltransferase 1, liver isoform
  • Scientific Description
    Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in triglyceride metabolism.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CPT1A using anti-CPT1A antibody (A00917-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT-4 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPT1A antigen affinity purified polyclonal antibody (Catalog # A00917-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPT1A at approximately 88 kDa. The expected band size for CPT1A is at 88 kDa.
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