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Goat anti-CPT1A Antibody

EB07419
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCPT1A
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-CPT1A Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID1374
  • Target name
    CPT1A
  • Target description
    carnitine palmitoyltransferase 1A
  • Target synonyms
    CPT I, CPT1, CPT1-L, CPTI-L, L-CPT1, carnitine O-palmitoyltransferase 1, liver isoform, carnitine palmitoyltransferase 1A (liver), carnitine palmitoyltransferase I, liver, succinyltransferase CPT1A
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001867.2; NP_001027017.1. Purification: Antigen affinity purified. Names and symbols: CPT1A; carnitine palmitoyltransferase 1A (liver) ; CPT1; CPT1-L; L-CPT1 ; carnitine palmitoyltransferase 1A; carnitine palmitoyltransferase I; carnitine palmitoyltransferase I, liver
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CPT1A using anti-CPT1A antibody (A00917-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human RT-4 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPT1A antigen affinity purified polyclonal antibody (Catalog # A00917-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPT1A at approximately 88 kDa. The expected band size for CPT1A is at 88 kDa.
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