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ProductsBack/CPT1B antibody [C1C2], Internal

CPT1B antibody [C1C2], Internal

GTX117231
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetCPT1B
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Overview

  • Supplier
    GeneTex
  • Product Name
    CPT1B antibody [C1C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.8 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1375
  • Target name
    CPT1B
  • Target description
    carnitine palmitoyltransferase 1B
  • Target synonyms
    carnitine O-palmitoyltransferase 1, muscle isoform; carnitine O-palmitoyltransferase 1B; carnitine palmitoyltransferase 1B (muscle); carnitine palmitoyltransferase I-like protein; CPT1M; CPT1-M; CPTI; CPTI-M; MCCPT1; MCPT1; M-CPT1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92523
  • Protein Name
    Carnitine O-palmitoyltransferase 1, muscle isoform
  • Scientific Description
    The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and one of the transcripts is bicistronic. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Cold exposure alters lipid metabolism of skeletal muscle through HIF-1alpha-induced mitophagy.
    Read more
  • Gan-Jiang-Ling-Zhu decoction alleviates hepatic steatosis in rats by the miR-138-5p/CPT1B axis. Dang Y et al., 2020 Jul, Biomed Pharmacother
    Read more
  • High density lipoprotein (HDL) reverses palmitic acid induced energy metabolism imbalance by switching CD36 and GLUT4 signaling pathways in cardiomyocyte. Wen SY et al., 2017 Nov, J Cell Physiol
    Read more
  • Cardiac-specific down-regulation of carnitine palmitoyltransferase-1b (CPT-1b) prevents cardiac remodeling in obese mice. Zhang Y et al., 2016 Dec, Obesity (Silver Spring)
    Read more

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Figure 1. Western blot analysis of CPT1B using anti-CPT1B antibody (A03558). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPT1B antigen affinity purified polyclonal antibody (Catalog # A03558) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPT1B at approximately 88 kDa. The expected band size for CPT1B is at 88 kDa.
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