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Western Blot analysis of HepG2 cells using CPTI-M Polyclonal Antibody
Western Blot analysis of HepG2 cells using CPTI-M Polyclonal Antibody
Western Blot analysis of HepG2 cells using CPTI-M Polyclonal Antibody

CPT1B Antibody

CSB-PA006785
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCPT1B
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Overview

  • Supplier
    Cusabio
  • Product Name
    CPT1B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1375
  • Target name
    CPT1B
  • Target description
    carnitine palmitoyltransferase 1B
  • Target synonyms
    carnitine O-palmitoyltransferase 1, muscle isoform; carnitine O-palmitoyltransferase 1B; carnitine palmitoyltransferase 1B (muscle); carnitine palmitoyltransferase I-like protein; CPT1M; CPT1-M; CPTI; CPTI-M; MCCPT1; MCPT1; M-CPT1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92523
  • Protein Name
    Carnitine O-palmitoyltransferase 1, muscle isoform
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CPT1B using anti-CPT1B antibody (A03558). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPT1B antigen affinity purified polyclonal antibody (Catalog # A03558) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPT1B at approximately 88 kDa. The expected band size for CPT1B is at 88 kDa.
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