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Western Blot analysis of HepG2 cells using CPTI-M Polyclonal Antibody
Western Blot analysis of HepG2 cells using CPTI-M Polyclonal Antibody
Western Blot analysis of HepG2 cells using CPTI-M Polyclonal Antibody

CPT1B Antibody

CSB-PA006785
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCPT1B
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Overview

  • Supplier
    Cusabio
  • Product Name
    CPT1B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1375
  • Target name
    CPT1B
  • Target description
    carnitine palmitoyltransferase 1B
  • Target synonyms
    CPT1-M, CPT1M, CPTI, CPTI-M, M-CPT1, MCCPT1, MCPT1, carnitine O-palmitoyltransferase 1, muscle isoform, carnitine O-palmitoyltransferase 1B, carnitine palmitoyltransferase 1B (muscle), carnitine palmitoyltransferase I-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92523
  • Protein Name
    Carnitine O-palmitoyltransferase 1, muscle isoform
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CPT1B using anti-CPT1B antibody (A03558). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPT1B antigen affinity purified polyclonal antibody (Catalog # A03558) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPT1B at approximately 88 kDa. The expected band size for CPT1B is at 88 kDa.
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ReactivityHuman, Monkey, Mouse, Rat
TargetCPT1B
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Formalin-fixed and paraffin embedded human rectal carcinoma tissue labeled with Anti-CPT1B Polyclonal Antibody, Unconjugated (bs-5045R) at 1:200, followed by conjugation to the secondary antibody and DAB staining
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Product group Antibodies
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Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CPT1B(GTX117231) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Product group Antibodies
References
GeneTex
CPT1B antibody [C1C2], InternalGTX117231
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetCPT1B
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