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ProductsBack/CR2 / CD21 Antibody (clone 257, Preservative Free)

CR2 / CD21 Antibody (clone 257, Preservative Free)

LS-C818870
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetCR2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CR2 / CD21 Antibody (clone 257, Preservative Free)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flo (1:25 - 1:100) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    257
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1380
  • Target name
    CR2
  • Target description
    complement C3d receptor 2
  • Target synonyms
    C3DR, CD21, CR, CVID7, SLEB9, complement receptor type 2, EBV receptor, complement component (3d/Epstein Barr virus) receptor 2, complement component 3d receptor 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD21/CR2 using anti-CD21/CR2 antibody (A01632-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse Raw264.7 whole cell lysates, Lane 2: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD21/CR2 antigen affinity purified polyclonal antibody (Catalog # A01632-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD21/CR2 at approximately 100,150 kDa. The expected band size for CD21/CR2 is at 113 kDa.
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