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ProductsBack/CRALBP antibody [B2]
IHC-Fr analysis of mouse retina sections using GTX15051 CRALBP antibody [B2].
IHC-Fr analysis of mouse retina sections using GTX15051 CRALBP antibody [B2].
IHC-Fr analysis of mouse retina sections using GTX15051 CRALBP antibody [B2].

CRALBP antibody [B2]

GTX15051
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityBovine, Human, Mouse, Primate, Rat
TargetRLBP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CRALBP antibody [B2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.02 microg/ml. ICC/IF: 1 microg/ml. IHC-Fr: 1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    B2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6017
  • Target name
    RLBP1
  • Target description
    retinaldehyde binding protein 1
  • Target synonyms
    CRALBP, retinaldehyde-binding protein 1, cellular retinaldehyde-binding protein, cellular retinaldehyde-binding protein-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP12271
  • Protein Name
    Retinaldehyde-binding protein 1
  • Scientific Description
    The protein encoded by this gene is a 36-kD water-soluble protein which carries 11-cis-retinaldehyde or 11-cis-retinal as physiologic ligands. It may be a functional component of the visual cycle. Mutations of this gene have been associated with severe rod-cone dystrophy, Bothnia dystrophy (nonsyndromic autosomal recessive retinitis pigmentosa) and retinitis punctata albescens. [provided by RefSeq, Jul 2008]
  • Reactivity
    Bovine, Human, Mouse, Primate, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

References

  • Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix. Felemban M et al., 2018 Jul 1, Acta Biomater
    Read this paper

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Figure 1. Western blot analysis of RLBP1 using anti-RLBP1 antibody (A05421-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RLBP1 antigen affinity purified polyclonal antibody (Catalog # A05421-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RLBP1 at approximately 36 kDa. The expected band size for RLBP1 is at 36 kDa.
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